Figure 1.

Overview of the fluorescence difference in-gel electrophoresis method: Shown are the main approaches used in fluorescence gel-based proteomics, whereby 2-dye or 3-dye difference in-gel electrophoresis (DIGE) is most commonly applied for studying global changes in complex protein populations. In the case of muscular dystrophy research, normal and dystrophic specimens are differentially labeled with CyDyes prior to electrophoresis, then proteins separated based on the unique combination of their isoeletric point and molecular masses and finally image analysis used to evaluate differential expression pattern of fluorescently labeled proteins.