Table 1.

List of identified proteins that exhibit a drastic change in abundance in the aged mdx diaphragm as revealed by 2D-DIGE analyses.

Spot No.	Protein Name	Accession No.	MS/MS Score	pI	Da	Number of peptides	Coverage (%)	Fold change
1	Dermatopontin	NP_062733	73	4.7	24,559	5	28	5.9
2	Dermatopontin	NP_062733	111	4.7	24,559	7	37	4.4
3	Dermatopontin	NP_062733	100	4.7	24,559	6	26	3.4
4	Heat shock protein HspB7 (beta7, cvHsp)	NP_038896	87	5.95	18,681	5	38	3.3
5	Myosin light chain 6B	NP_758463	181	5.41	22,851	14	74	3.1
6	Tropomyosin, beta chain isoform 1	NP_033442	319	4.66	32,933	22	61	2.9
7	Unnamed protein product	BAE35818	286	5.78	70,776	15	30	2.8
8	Myosin light chain 1/3, isoform 1f, muscle	NP_067260	97	4.98	20,697	2	12	2.7
9	Tropomyosin, beta chain isoform 1	NP_033442	107	4.66	32,933	11	35	2.7
10	Actin, beta (aa 27–375)	CAA27396	124	5.78	39,451	9	21	2.6
11	Heat shock protein Hsp70 cognate	AAA37869	110	5.37	71,025	11	23	2.6
12	Mitochondrial stress-70 protein	BAA04493	57	5.91	73,773	1	1	2.6
13	Actin, gamma, smooth muscle	AAC52237	81	5.36	43,258	5	16	2.6
14	Nebulin (fragment)	AAF59979	53	9.19	87,694	4	7	2.5
15	Lumican	AAB35361	103	5.84	38,733	2	5	2.4
16	Prohibitin	NP_032857	350	5.57	29,860	15	69	2.4
17	Unnamed protein product	BAC34145	129	5.75	70,766	8	14	2.4
18	Transferrin	AAL34533	168	6.92	78,832	19	24	2.3
19	Annexin A5	NP_033803	249	4.83	35,788	16	45	2.3
20	Alpha-fetoprotein	AAA37190	112	5.47	48,819	2	6	2.2
21	Heat shock protein Hsp70 cognate	AAA37869	110	5.37	71,025	11	23	2.2
22	Heat shock protein Hsp70 cognate	AAA37869	77	5.37	71,025	4	6	2.2
23	Heat shock protein 8	AAH66191	110	5.28	71,060	11	23	2.2
24	Mitochondrial stress-70 protein	BAA04493	71	5.91	73,773	5	9	2.1
25	Fmod protein	AAH52673	92	5.56	46,637	2	4	2.1
26	Unnamed protein product	BAC34145	254	5.75	70,766	8	17	2.1
27	78 kDa glucose-regulated protein	BAA11462	112	5.09	72,528	7	15	2.0
28	Myosin light chain 1/3, isoform 1f, muscle	NP_067260	222	4.98	20,697	15	63	−2.0
29	Parvalbumin, alpha	NP_038673	156	5.02	11,923	6	57	−2.0
30	Malate dehydrogenase	AAA39509	90	8.93	36,052	3	11	−2.1
31	ATP synthase, beta-subunit	AAB86421	98	5.14	56,344	2	4	−2.1
32	Tropomyosin, beta chain	gi|11875203	180	4.66	32,933	11	32	−2.1
33	Alpha-fetoprotein	AAA37190	95	5.47	48,819	2	5	−2.1
34	Isocitrate dehydrogenase subunit alpha	NP_083849	242	6.27	40,077	6	20	−2.1
35	L-lactate dehydrogenase B chain	NP_032518	54	5.7	36,839	5	12	−2.1
36	Actin, alpha, cardiac	gi|387090	51	5.23	42,048	2	7	−2.1
37	Coiled-coil-helix-coiled-coil-helix domain-containing protein 3, mitochondrial	NP_079612	80	8.56	26,550	5	24	−2.1
38	Cu/Zn superoxide dismutase	1513495A	147	6.03	15,926	5	41	−2.1
39	Malate dehydrogenase mitochondrial	NP_032643	256	8.93	36,053	12	45	−2.1
40	Peroxiredoxin Prdx1	NP_035164	97	8.26	22,394	11	47	−2.1
41	Malate dehydrogenase mitochondrial	NP_032643	343	8.93	36,053	15	56	−2.1
42	Cytochrome c oxidase, subunit Va	CAA34085	125	6.08	16,252	8	48	−2.2
43	ATP synthase, mitochondrial F0 complex	AAH16547	148	5.52	18,810	5	47	−2.2
44	Heat shock protein Hsp beta-6	NP_001012401	130	5.64	17,568	6	51	−2.2
45	Creatine kinase, M-type	NP_031736	276	6.58	43,250	9	27	−2.2
46	Malate dehydrogenasemitochondrial	NP_032643	243	8.93	36,053	9	36	−2.2
47	Troponin C, skeletal muscle	NP_033420	238	4.07	18,156	3	28	−2.2
48	Troponin C, skeletal muscle	NP_033420	774	4.07	18,156	8	53	−2.3
49	Malate dehydrogenase 2, mitochondrial	ABD77283	365	7.7	32,121	7	28	−2.3
50	Myoglobin	NP_038621	61	7.07	17,117	1	9	−2.3
51	Myosin light chain 1/3, isoform 1f, muscle	NP_067260	93	4.98	20,697	4	17	−2.3
52	Creatine kinase, M-type	NP_031736	297	6.58	43,250	10	28	−2.3
53	Fatty acid-binding protein FABP3	NP_034304	157	6.11	14,810	6	48	−2.3
54	Calcium-binding protein NCS-1-like	XP_003945963	48	6.49	12,957	1	17	−2.4
55	Electron transferring flavoprotein, alpha	AAH03432	449	8.62	35,366	11	44	−2.4
56	Isocitrate dehydrogenase subunit alpha	NP_083849	167	6.27	40,077	6	19	−2.4
57	Malate dehydrogenase 2, mitochondrial	ABD77283	85	7.7	32,121	2	8	−2.4
58	Creatine kinase, M-type	NP_031736	404	6.58	43,250	11	33	−2.4
59	ATP synthase, subunit d, mitochondrial	NP_082138	168	5.52	18,796	11	70	−2.4
60	Electron transfer flavoprotein, subunit alpha	NP_663590	195	8.62	35,336	8	39	−2.5
61	Cofilin-2	NP_031714	158	7.66	18,814	5	36	−2.5
62	Adenylate kinase, isoenzyme AK1	NP_067490	104	5.7	23,334	6	36	−2.5
63	ATP synthase, beta-subunit	AAB86421	301	5.14	56,344	6	16	−2.6
64	Tropomyosin, beta chain	gi|11875203	129	4.66	32,920	10	25	−2.6
65	Myosin light chain MLC2, skeletal muscle	NP_058034	93	4.82	19,059	2	8	−2.7
66	Fumarate hydratase 1	AAH06048	173	9.12	54,568	6	17	−2.8
67	Myosin light chain 1/3, isoform 1f, muscle	NP_067260	201	4.98	20,697	11	53	−2.9
68	Ubiquinone biosynthesis protein COQ9	NP_080728	150	5.6	35,235	5	21	−3.0
69	Calsequestrin, skeletal muscle	AAC63616	143	3.93	45,619	8	22	−3.1
70	Creatine kinase, M-type	NP_031736	505	6.58	43,250	10	31	−3.1
71	Myoglobin	NP_038621	124	7.07	17,117	6	48	−3.1
72	Myoglobin	NP_038621	59	7.07	17,117	1	11	−3.2
73	Myosin light chain MLC2, skeletal muscle	NP_058034	267	4.82	19,059	15	68	−3.4
74	Creatine kinase, M-type	NP_031736	266	6.58	43,250	11	31	−3.5
75	Myosin light chain 1/3, isoform 1f, muscle	NP_067260	87	4.98	20,697	2	12	−3.6
76	Myoglobin	NP_038621	91	7.07	17,117	2	21	−3.7
77	Malate dehydrogenase	AAA39509	126	8.93	36,052	5	20	−3.7
78	Fatty acid-binding protein FABP3	NP_034304	426	6.11	14,810	11	77	−3.7
79	Myoglobin	NP_038621	129	7.07	17,117	6	46	−4.0
80	Parvalbumin, alpha	NP_038673	285	5.02	11,923	6	57	−4.0
81	Myoglobin	NP_038621	89	7.07	17,117	6	41	−4.3
82	Fatty acid-binding protein FABP3	NP_034304	188	6.11	14,810	7	63	−5.3
83	Fatty acid-binding protein FABP3	NP_034304	532	6.11	14,810	9	71	−5.3
84	Parvalbumin alpha	NP_038673	431	5.02	11,923	8	57	−10.5