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. 2012 Oct 1;1(3):495–507. doi: 10.3390/biology1030495

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© 2012 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Main steps of MmeI-assisted cassette-PCR GW and SBS-Illumina association strategy. Blue and white bars correspond to known and unknown sequences, respectively. An open vertical arrow indicates the MmeI recognition site. The symbol indicates the MmeI digestion site. A black horizontal arrow indicates adapter specific primers; a blue arrow represents primers designed in the known region. Barcodes and Illumina sequencing traits of longer amplification primers are indicated in the figure.

Inline graphic — Main steps of MmeI-assisted cassette-PCR GW and SBS-Illumina association strategy. Blue and white bars correspond to known and unknown sequences, respectively. An open vertical arrow indicates the MmeI recognition site. The symbol indicates the MmeI digestion site. A black horizontal arrow indicates adapter specific primers; a blue arrow represents primers designed in the known region. Barcodes and Illumina sequencing traits of longer amplification primers are indicated in the figure.