Skip to main content
PMC home page
The EMBO Journal logoLink to The EMBO Journal
. 1989 May;8(5):1507–1515. doi: 10.1002/j.1460-2075.1989.tb03535.x

Two gene members of the murine HOX-5 complex show regional and cell-type specific expression in developing limbs and gonads.

P Dollé 1, D Duboule 1
PMCID: PMC400982  PMID: 2569970

Abstract

This study reports the expression domains of two murine HOX gene members of the HOX-5 complex (Hox-5.2, Hox-5.3). These two genes have very similar homeodomain sequences, as well as temporal and spatial specificities of expression. They are both expressed at very posterior levels in the central nervous system, in sclerotome derivatives and in a few internal organs. In addition to these expression domains which are shared with other HOX genes, transcripts from both Hox-5.2 and Hox-5.3 are present at high levels in developing limbs. After an early homogeneous expression in mesodermal limb bud cells, transcription becomes restricted to cartilage-differentiating cells. In addition, Hox-5.2 is a marker for gonadal development. The possible involvement of such genes during inductive processes or organogenesis is discussed.

Full text

PDF
1515

Images in this article

1508Image
on p.1508
1509Image
on p.1509
1511Image
on p.1511
1512Image
on p.1512
1513Image
on p.1513

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Awgulewitsch A., Utset M. F., Hart C. P., McGinnis W., Ruddle F. H. Spatial restriction in expression of a mouse homoeo box locus within the central nervous system. 1986 Mar 27-Apr 2Nature. 320(6060):328–335. doi: 10.1038/320328a0. [DOI] [PubMed] [Google Scholar]
  2. Breier G., Dressler G. R., Gruss P. Primary structure and developmental expression pattern of Hox 3.1, a member of the murine Hox 3 homeobox gene cluster. EMBO J. 1988 May;7(5):1329–1336. doi: 10.1002/j.1460-2075.1988.tb02948.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Colberg-Poley A. M., Voss S. D., Chowdhury K., Stewart C. L., Wagner E. F., Gruss P. Clustered homeo boxes are differentially expressed during murine development. Cell. 1985 Nov;43(1):39–45. doi: 10.1016/0092-8674(85)90010-8. [DOI] [PubMed] [Google Scholar]
  4. Cox K. H., DeLeon D. V., Angerer L. M., Angerer R. C. Detection of mrnas in sea urchin embryos by in situ hybridization using asymmetric RNA probes. Dev Biol. 1984 Feb;101(2):485–502. doi: 10.1016/0012-1606(84)90162-3. [DOI] [PubMed] [Google Scholar]
  5. Desplan C., Theis J., O'Farrell P. H. The sequence specificity of homeodomain-DNA interaction. Cell. 1988 Sep 23;54(7):1081–1090. doi: 10.1016/0092-8674(88)90123-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dony C., Gruss P. Proto-oncogene c-fos expression in growth regions of fetal bone and mesodermal web tissue. Nature. 1987 Aug 20;328(6132):711–714. doi: 10.1038/328711a0. [DOI] [PubMed] [Google Scholar]
  7. Dony C., Gruss P. Specific expression of the Hox 1.3 homeo box gene in murine embryonic structures originating from or induced by the mesoderm. EMBO J. 1987 Oct;6(10):2965–2975. doi: 10.1002/j.1460-2075.1987.tb02602.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Duboule D., Baron A., Mähl P., Galliot B. A new homeo-box is present in overlapping cosmid clones which define the mouse Hox-1 locus. EMBO J. 1986 Aug;5(8):1973–1980. doi: 10.1002/j.1460-2075.1986.tb04452.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Duboule D., Dollé P. The structural and functional organization of the murine HOX gene family resembles that of Drosophila homeotic genes. EMBO J. 1989 May;8(5):1497–1505. doi: 10.1002/j.1460-2075.1989.tb03534.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Featherstone M. S., Baron A., Gaunt S. J., Mattei M. G., Duboule D. Hox-5.1 defines a homeobox-containing gene locus on mouse chromosome 2. Proc Natl Acad Sci U S A. 1988 Jul;85(13):4760–4764. doi: 10.1073/pnas.85.13.4760. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gaunt S. J. Homoeobox gene Hox-1.5 expression in mouse embryos: earliest detection by in situ hybridization is during gastrulation. Development. 1987 Sep;101(1):51–60. [PubMed] [Google Scholar]
  12. Gaunt S. J., Miller J. R., Powell D. J., Duboule D. Homoeobox gene expression in mouse embryos varies with position by the primitive streak stage. Nature. 1986 Dec 18;324(6098):662–664. doi: 10.1038/324662a0. [DOI] [PubMed] [Google Scholar]
  13. Graham A., Papalopulu N., Lorimer J., McVey J. H., Tuddenham E. G., Krumlauf R. Characterization of a murine homeo box gene, Hox-2.6, related to the Drosophila Deformed gene. Genes Dev. 1988 Nov;2(11):1424–1438. doi: 10.1101/gad.2.11.1424. [DOI] [PubMed] [Google Scholar]
  14. Hart C. P., Fainsod A., Ruddle F. H. Sequence analysis of the murine Hox-2.2, -2.3, and -2.4 homeo boxes: evolutionary and structural comparisons. Genomics. 1987 Oct;1(2):182–195. doi: 10.1016/0888-7543(87)90011-5. [DOI] [PubMed] [Google Scholar]
  15. Hoey T., Levine M. Divergent homeo box proteins recognize similar DNA sequences in Drosophila. Nature. 1988 Apr 28;332(6167):858–861. doi: 10.1038/332858a0. [DOI] [PubMed] [Google Scholar]
  16. Holland P. W., Hogan B. L. Expression of homeo box genes during mouse development: a review. Genes Dev. 1988 Jul;2(7):773–782. doi: 10.1101/gad.2.7.773. [DOI] [PubMed] [Google Scholar]
  17. Holland P. W., Hogan B. L. Spatially restricted patterns of expression of the homeobox-containing gene Hox 2.1. during mouse embryogenesis. Development. 1988 Jan;102(1):159–174. doi: 10.1242/dev.102.1.159. [DOI] [PubMed] [Google Scholar]
  18. Le Mouellic H., Condamine H., Brûlet P. Pattern of transcription of the homeo gene Hox-3.1 in the mouse embryo. Genes Dev. 1988 Jan;2(1):125–135. doi: 10.1101/gad.2.1.125. [DOI] [PubMed] [Google Scholar]
  19. McGinnis W., Levine M. S., Hafen E., Kuroiwa A., Gehring W. J. A conserved DNA sequence in homoeotic genes of the Drosophila Antennapedia and bithorax complexes. 1984 Mar 29-Apr 4Nature. 308(5958):428–433. doi: 10.1038/308428a0. [DOI] [PubMed] [Google Scholar]
  20. Melton D. A., Krieg P. A., Rebagliati M. R., Maniatis T., Zinn K., Green M. R. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 1984 Sep 25;12(18):7035–7056. doi: 10.1093/nar/12.18.7035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Müller M., Affolter M., Leupin W., Otting G., Wüthrich K., Gehring W. J. Isolation and sequence-specific DNA binding of the Antennapedia homeodomain. EMBO J. 1988 Dec 20;7(13):4299–4304. doi: 10.1002/j.1460-2075.1988.tb03328.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Oliver G., Wright C. V., Hardwicke J., De Robertis E. M. A gradient of homeodomain protein in developing forelimbs of Xenopus and mouse embryos. Cell. 1988 Dec 23;55(6):1017–1024. doi: 10.1016/0092-8674(88)90246-2. [DOI] [PubMed] [Google Scholar]
  23. Regulski M., Harding K., Kostriken R., Karch F., Levine M., McGinnis W. Homeo box genes of the Antennapedia and bithorax complexes of Drosophila. Cell. 1985 Nov;43(1):71–80. doi: 10.1016/0092-8674(85)90013-3. [DOI] [PubMed] [Google Scholar]
  24. Rubin M. R., King W., Toth L. E., Sawczuk I. S., Levine M. S., D'Eustachio P., Nguyen-Huu M. C. Murine Hox-1.7 homeo-box gene: cloning, chromosomal location, and expression. Mol Cell Biol. 1987 Oct;7(10):3836–3841. doi: 10.1128/mcb.7.10.3836. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Sharpe C. R., Fritz A., De Robertis E. M., Gurdon J. B. A homeobox-containing marker of posterior neural differentiation shows the importance of predetermination in neural induction. Cell. 1987 Aug 28;50(5):749–758. doi: 10.1016/0092-8674(87)90333-3. [DOI] [PubMed] [Google Scholar]

Articles from The EMBO Journal are provided here courtesy of Nature Publishing Group

RESOURCES