Figure 7. Misfolded Sec61-2-GFP asymmetry depends on the cER diffusion barrier.

(A) Images of Sec61-2-GFP of a budding ubc7Δ (up) and a budding ubc7Δ bud1Δ (bottom) mutant cell. The arrow indicates the mother cell and the arrowhead the growing bud. Scale bar = 2 µm. (B) Distribution of asymmetry indices measured at the last frame prior to anaphase in individual cells of indicated genotype (note that the quantification of Sec61-2-GFP ubc7Δ is the same as in Figure 1G). ***p<0.0001 (t test).

DOI: http://dx.doi.org/10.7554/eLife.01883.009