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. 2013 Feb 28;2(1):341–355. doi: 10.3390/biology2010341

Figure 3.

Figure 3

(A) Experimental flow for examining whether enrichment of cells with high transgene expression can be achieved with IB4SAP treatment. Abbreviations are the same in Figure 2A. (B) Micrographs taken under light + UV illumination. (a), The cells obtained one week after SAP treatment; and (b) and (c), the emerging colonies obtained two weeks after IB4SAP treatment. In (a), a mixture of fluorescent cells (successfully transfected with pCEIEnd) and nonfluorescent untransfected cells is indicated by an asterisk. The cells in (b) and (c) are from two independent colonies. Bar = 100 μm. (C) Dissociated cells isolated from a colony described in (c) of B. These cells were then stained with AF594-IB4. The arrows indicate the isolated cells in (a) showing bright green fluorescence (b) but negative for lectin staining (c). (a), photograph taken under light; (b) and (c), photographs taken under light + UV illumination. Bar = 100 μm.