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. Author manuscript; available in PMC: 2014 May 5.
Published in final edited form as: Mol Microbiol. 2013 Nov 21;91(1):57–65. doi: 10.1111/mmi.12439

Figure 4.

Figure 4

Early triggering by S105A52F or by S105 wt with the addition of KCN. E. coli MC4100 ΔtonA λCamΔSR carrying either pS105RamRzamRz1am or an isogenic plasmid with the S105A52F allele were thermally induced and KCN was added to the former culture 15 minutes later. Cells were plunge frozen and imaged as described for Fig. 2. (A) Number of holes/cell observed and (B) hole size distribution for S105A52F. (C) Number of holes/cell and (D) hole size distribution for wt S105 prematurely triggered with KCN.