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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Alcohol Clin Exp Res. 2014 Mar 3;38(5):1216–1226. doi: 10.1111/acer.12373

Figure 4.

Figure 4

Dietary cholesterol protects against AICAC via accumulation of cholesterol in arterial tissue. (A) Free cholesterol content (in µg per mg of total tissue protein) in de-endothelialized arteries of rats receiving control (n=6) vs. high-cholesterol diet (n=6). (B) Outline of experimental design aimed to regain AICAC by removal of accumulated cholesterol from de-endothelialized, pressurized cerebral arteries of rats on high-cholesterol diet. MβCD: methyl-beta-cyclodextrin, PSS: physiologic saline solution. Representative trace of cerebral artery diameter (top) shows augmented AICAC after removal of accumulated cholesterol using MβCD-containing PSS. Representative trace of cerebral artery diameter (bottom) shows lack of augmentation of AICAC after artery perfusion with MβCD-free PSS. (C) Averaged data comparing AICAC by EtOH I (n=6) with AICAC after MβCD removal of accumulated cholesterol (n=3), and after artery perfusion with MβCD-free PSS (n=3). *p<0.05, when compared to AICAC following removal of accumulated cholesterol from de-endothelialized, pressurized cerebral arteries of rats on high-cholesterol diet.