Skip to main content
. Author manuscript; available in PMC: 2014 May 5.
Published in final edited form as: Chemistry. 2013 Oct 14;19(47):15872–15878. doi: 10.1002/chem.201302188

Table 2.

Enzymatic ligation of selected tRNASec variants.

tRNA mimic[a] Mwcalcd Mwobs[d] Yield[e] [nmol]
Abu(p)-tRNASec (90 nt)[b] 29095 29096 1.0
Abu(p)-tRNAU1ASec (93 nt)[c] 30028 30028 0.5
Ser(p/NαCH3)-tRNASec (93 nt)[c] 30044 30043 4.0
[a]

For sequences see Figure 2.

[b]

Ligation of 38, 33, and 19 nt long fragments.

[c]

Ligation of 39, 35, and 19 nt long fragments.

[d]

Measured by LC-ESI mass spectrometry.

[e]

Yield of purified, isolated tRNA.