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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Nat Methods. 2014 Feb 16;11(4):429–435. doi: 10.1038/nmeth.2845

Figure 4. In vitro specificity as a function of TALEN length.

Figure 4

The enrichment value of on-target (zero mutation) and off-target sequences containing one to six mutations are shown for CCR5B TALENs of varying TALE repeat array lengths with EL/KK FokI domains. The TALENs targeted DNA sites of 32 bp (L16+R16), 29 bp (L16+R13 or L13+R16), 26 bp (L16+R10 or L13+R13 or L10+R16), 23 bp (L13+R10 or L10+R13) or 20 bp (L10+R10) in length.