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. Author manuscript; available in PMC: 2014 Nov 18.
Published in final edited form as: Curr Biol. 2013 Nov 7;23(22):2319–2324. doi: 10.1016/j.cub.2013.09.061

Figure 4. Survival and phagocytosis are affected when blood cell amino acid transport is modulated.

Figure 4

Wildtype blood cells in hemolymph were incubated ex vivo with fluorescein S.aureus bioparticles in PBS or 5mM THA (A), or media plus nothing, glutamate, cystine, or glutamate and cystine (B). After 30 minutes the number of bioparticles/cell was counted in approximately 20 cells per group in each experiment. (C) Representative survival curves of wt, gb and eaat1 flies after injection of S.aureus (OD 0.05). n = 30 flies. Quantification of the phagocytosis of fluorescein-labeled S.aureus bioparticles in wt and gb flies (D), wt and eaat1 flies (E), and wt,polyph, hml/wt and polyph; hml>eaat1 flies (F). Approximately 6 flies per genotype were used in each experiment. All experiments were performed at least in triplicate. Error bars, ±SE. *p<0.05, ** p<0.01, ns = not significantly different.