Table 2.
T Cell Subsets Analyzed in the Study, Grouped According to Eight Different Categories
| T cell subset category (antibody panel) | T cell subset |
|---|---|
| Differentiation stage of CD4 and CD8 cells (CD3-FITC/CD27-PE/CD45RA-ECD/CD8-PECy5/CD4-PECy7) | CD4 and CD8 cells (total) |
| Naive cells (CD45RA+CD27+) | |
| Central memory cells (CD45RA−CD27+) | |
| Effector memory cells (CD45RA−CD27−) | |
| Effector cells (CD45RA+CD27−) | |
| Activation of CD4 and CD8 T cells (including naive, central memory, effector memory, and effector cells) (HLADR-FITC/CD27-PE/CD45RA-ECD/CD38-PECy5/CD4-PECy7 or CD8- PECy7) | CD38+DR− |
| CD38+DR+ | |
| CD38−DR+ | |
| Thymic function (CD31) (CD31-FITC/CD27-PE/CD45RA-ECD/CD8-PECy5/CD4-PECy7) | CD31 expression on CD4 and CD8 cells (including naive, central memory, effector memory, and effector cells) |
| Expression of CD127 (CD127-FITC/CD27-PE/CD45RA-ECD/CD8-PECy5/CD4-PECy7) | CD127 expression on CD4 and CD8 cells (including naive, central memory, effector memory, and effector cells) |
| Senescence (CD57) (CD57-FITC/CD27-PE/CD45RA-ECD/CD8-PECy5/CD4-PECy7) | CD57 expression on CD4 and CD8 cells (including naive, central memory, effector memory, and effector cells) |
| Exhaustion (PD-1 and Tim-3) of CD3, CD4, and CD8 T cells (CD4-FITC/Tim-3-PE/CD3-ECD/CD8-PECy5/PD-1-biotin/streptavidin-PECy7) | PD-1+Tim-3− |
| PD-1+Tim-3+ | |
| PD-1−Tim-3+ | |
| Apoptosis (annexin binding) (annexin-FITC/CD27-PE/CD45RA-ECD/CD8-PECy5/CD4-PECy7) | Annexin+ binding on CD4 and CD8 cells (including naive, central memory, effector memory, and effector cells) |
| T regulatory cells (FoxP3-FITC/CD127-PE/CD8-ECD/CD25-PECy5/CD4-PECy7) | CD4+CD25+CD127−FoxP3+ cells |