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. Author manuscript; available in PMC: 2014 May 5.
Published in final edited form as: Dev Cell. 2002 Jun;2(6):713–720. doi: 10.1016/s1534-5807(02)00154-5

Figure 7. Responses to Dietary Bile Acids, Cholestyramine, and FXR Agonist GW4064.

Figure 7

(A) Mice of different Shp genotypes (n = 4–6 each) were fed the indicated diets (normal versus chow supplemented with 0.25% [w/w] cholic acid) or gavaged with the FXR agonist GW4064 (100 mg/kg body weight, q.d.) for 5 days. Livers were dissected, total RNA prepared and pooled, and cholesterol 7α-hydroxylase (CYP7A1) and sterol 12α-hydroxylase (CYP8B1) mRNA levels determined by real-time reverse transcriptase PCR (upper panel) in individual animals. The numbers shown are the averages of triplicate determinations for each mRNA. Hepatic enzyme levels (AST, aspartate aminotransferase; ALT, alanine aminotransferase) were determined in pooled plasmas from the same groups of mice (lower panel).

(B) Animals were fed chow diet supplemented with nothing or cholestyramine (2%, w/w) for 5 days. CYP7A1 and CYP8B1 mRNA levels were quantitated as described in (A).