Table 1. Primers for target genes.

Name	Size (bp)	Primers	Locus number
Cbfa-1	200	Forward: 5′-GCAAGGTTCAACGATCTGAG-3′ Reverse: 5′-GAGGCGGTCAGAGAACAAAC-3′	NM_001024630.3
OCN	335	Forward: 5′-CAGCCACCGAGACACCAT-3′ Reverse: 5′-CCAGCAGAGCGACACCCTA-3′	NM_199173.3
OPG	207	Forward: 5′-CCTTGCCCTGACCACTAC-3′ Reverse: 5′-TTGCACCACTCCAAATCC-3′	NM_002546.3
HIF-1α	408	Forward: 5′-AAACCACCTATGACCTGC-3′ Reverse: 5′-GTCGTGCTGAATAATACCACTC-3′	NM-001530.3
Beta-actin	302	Forward: 5′-TCAGGAGGAGCAATGATCTTG-3′ Reverse: 5′-TCCTCCCTGGAGA AGAGCTA-3′	NM_031144.2

Cbfa-1, core-binding factor a 1; HIF-1α, hypoxia inducible factor 1α, OPG: osteoprotegerin; OCN, osteocalcin.