Table 1. Target sequences and the design of molecular beacons.

Gene	Sequences
Survivin	Target: 5′-ATGGGTGCCCCGACGTTGCCCCCTGCC TGGCAGCCCTTTCTC aagg ACCACCGCATCTCTAC ATTCAAGAACTGGCCC-3′ Donor MB: 5′-/Cy3/GAGAAAGGGCTGCCATTCTC/BHQ-2/-3′ Acceptor MB: 5′-/BHQ-3/ACCACGTAGAGATGCGGTGGT/Cy5/-3′ Donor MB: 5′-/FAM/GAGAAAGGGCTGCCATTCTC/BHQ-2/-3′ Acceptor MB: 5′-/BHQ-3/ACCACGTAGAGATGCGGTGGT/Cy3/-3′
Random	Target: 5′-ATCGGTGCGCTTGTCG-3′ Donor MB: 5′-/Cy3/CACGTCGACAAGCGCACCGATACGTG/BHQ-2/-3′ Acceptor MB: 5′-/BHQ-3/ACGTGCGACAAGCGCACCGATCACGT/Cy5/-3′

Molecular beacon (MB): underlined bases, bases added to create the stem domain. Target: lowercase bold, bases between two target sequences of the donor and acceptor beacon¹¹.