Figure 3.

Effect of magnesium sulphate (MgSO₄) on the concentration-response of acetylcholine (ACh) on adult muscle-type acetylcholine receptor. (A) Inward currents evoked by superfusion of 1, 10, 50, 100, and 500 μmol/L ACh alone (as indicated by horizontal bars) during control conditions and co-application of 3 and 30 mmol/L MgSO₄. MgSO₄ was pre-applied for 60 s before co-application with 1, 10, 50, 100, and 500 μmol/L ACh. (B) The effect on inward currents mediated by adult muscle-type acetylcholine receptor over a range of acetylcholine concentrations in the presence of 0, 3, and 30 mmol/L magnesium sulphate (MgSO₄). Data points represent the mean±SD of 5 HEK293 cells.