Effect of vecuronium (VEC) on the concentration-response of acetylcholine (ACh) on adult muscle-type acetylcholine receptor. (A) Inward currents evoked by superfusion of 1, 10, 100, and 500 μmol/L ACh alone (as indicated by horizontal bars) during control conditions and co-application of 10 nmol/L vecuronium. Vecuronium was pre-applied for 60 s before co-application with 1, 10, 100, and 500 μmol/L ACh. (B) The effect on inward currents mediated by adult muscle-type acetylcholine receptor over a range of acetylcholine concentrations in the presence of 10 nmol/L vecuronium. Data points represent the mean±SD (error bars) of 5 HEK293 cells.