Figure 3.
Wogonin restored the ratio of Bcl-2 to Bax. A10 VSMCs were cultured in serum-free medium for 6 h and treated with 0.75 mmol/L of palmitate (PA) for 12 h, followed by a second treatment with or without 25 μmol/L of wogonin for an additional 12 h. (A) Bcl-2 and Bax protein expression. (B) Bar graph for the ratio of Bcl-2 to Bax. Western blot was performed to detect the expression levels of Bcl-2 and Bax. The figures are representative of at least three independent experiments with similar results. cP<0.01 compared with the control group. fP<0.01 compared with the palmitate-treated group.