Expressions of α4, α7, and β2 nAChR subunits at protein level in the rat brains. The nAChR α4 (A), α7 (B), and β2 (C) subunit proteins in the rat brains as well as β-actin (as an internal standard) were measured by Western blotting. For the calculation of Western blotting results, β-actin (a house-keeping gene) was used as an internal standard, and the resultant values (as compared with β-actin) were expressed as a percentage of the average value for controls (as 100%). bP<0.05 as compared to normal group and sham operation group. eP<0.05 as compared to model group as determined by an analysis of the variance ANOVA with Tukey-Kramer using SPSS15.0 software.