Table 1.

Parameters for Nuclear Quantitation From Aperio’s Image Analysis Tool Box

Parameter	Value
Version	9.1
View width	1,000
View height	1,000
Overlap size	100
Image zoom	1
Classifier	None
Classifier neighborhood	0
Averaging radius (pixels)	3
Curvature threshold	1
Segmentation type	1
Threshold type	2
Lower intensity threshold	0
Upper intensity threshold	200
Minimum nuclear size (pixels)	80
Maximum nuclear size (pixels)	1,200
Minimum roundness	0.1
Minimum compactness	0
Minimum elongation	0.1
Remove light objects	0
Weak (1+) threshold	190
Moderate (2+) threshold	1
Strong (3+) threshold	1
Black threshold	0
Edge trim	Weighted
Markup image type	Analysis
Nuclear red OD	0.730342
Nuclear green OD	0.574991
Nuclear blue OD	0.368763
Positive red OD	0.244583
Positive green OD	0.509334
Positive blue OD	0.825081
Color (3) red OD	0
Color (3) green OD	0
Color (3) blue OD	0
Clear area intensity	240
Use mode	Analysis/tuning
Classifier type	IHC nuclear
Classifier definition file	IHC nuclear training

IHC, immunohistochemistry; OD, optical density.