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. 2011 Nov 28;33(1):109–119. doi: 10.1038/aps.2011.145

Figure 7.

Figure 7

(A) Effects on the protein expression of H3K4me2, H3K9me2, H3K36me2, histone demethylase LSD1 and JMJD2B. RPMI8226 cells were treated with various concentrations (50, 100, 150 nmol/L) triptolide for 48 h, then cell lysates were subjected to SDS-PAGE followed by Western blotting with anti-H3K4me2, anti-H3K9me2, anti-H3K36me2, anti-LSD1, anti-JMJD2B antibodies. Each membrane was then stripped and reprobed with anti-β-actin antibody to confirm equal protein loading. (B) The statistical results of protein expression of H3K4me2, H3K9me2, H3K36me2, LSD1 and JMJD2B. Mean±SD. n=3. aP>0.05.bP<0.05 vs control.