Table 1. Primer sequences for ALP, OC, OPG, RANKL, ERα, ERβ, and S18.

Gene	Sequence	Fragment length (bp)	Accession number
ALP	F: 5′-CGAGCAGGAACAGAAGTTTGC-3′ R: 5′-TGGCCAAAAGGCAGTGAATAG-3′	105 bp	NM_013059
OC	F: 5′-ATGCCACTGCGTATTGGTTGA-3′ R: 5′-TCCGCTAGCTCGTCACAATTG-3′	122 bp	M25490
OPG	F: 5′-AGCTGGCACACGAGTGATGAA-3′ R: 5′-CACATTCGCACACTCGGTTGT-3′	106 bp	U94330
RANKL	F: 5′-GGAGGATTTTTCAAGCTCCGG-3′ R: 5′-TGAAAGCCCCAAAGTACGTCG-3′	103 bp	NM_057149
ERα	F: 5′-TGCGCAAGTGTTACGAAGTGG-3′ R: 5′-TTCGGCCTTCCAAGTCATCTC-3′	108 bp	NM_012689
ERβ	F: 5′-AAAAACTCACCGTCGAGCCTT-3′ R: 5′-GCTGAATACTCATGGCGGTTG-3′	124 bp	NM_012754
S18	F: 5′-CGGCTACCACATCCAAGGAA-3′ R: 5′-GCTGGAATTACCGCGGCT-3′	187 bp	M11188

ALP, alkaline phosphatase; OC, osteocalcin; OPG, osteoprotegerin; RANKL, receptor activator of nuclear factor κB ligand; ERα, estrogen receptor α ERβ, estrogen receptor β S18, ribosomal protein S18, a housekeeping gene.