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. 2011 Nov 21;33(1):120–126. doi: 10.1038/aps.2011.139

Figure 1.

Figure 1

Effects of EGCG on PAR2-AP- and factor VIIa-induced proliferation and migration of SW620 cells. (A) SW620 cells were plated at 5×104 cells in 96-well plates and pretreated with different concentrations of EGCG (0, 25, 50, 75, and 100 μg/mL) for 15 min, then incubated with PAR2-AP (100 μmol/L) or factor VIIa (10 nmol/L) for 24 h. The above EGCG was kept in media. The cell proliferation was measured using MTT colorimetric assay. n=6. Mean±SEM. eP<0.05, fP<0.01 vs PAR2-AP- or factor VIIa-stimulated alone. (B) The cells were pretreated with or without EGCG (100 μg/mL) for 15 min as described above and then incubated with PAR2-AP (100 μmol/L) or factor VIIa (10 nmol/L) for 8 h. The cell migratory potential was measured by modified Boyden Chambers as described in Methods. n=3. Mean±SEM. aP>0.05, cP<0.01 vs media only; eP<0.05, fP<0.01 vs PAR2-AP- or factor VIIa-stimulated alone.