Figure 3.

Effects of EGCG on caspase-7 expression in SW620 cells. SW620 cells (1×10⁶–1×10⁷) were stimulated with PAR2-AP (100 μmol/L) or factor VIIa (10 nmol/L) in the absence or presence of EGCG (100 μg/mL) for 2 h or 24 h. Then the total RNAs (2 h) and cell lysates (24 h) were collected for caspase-7 mRNA (A) and its protein (B) determination using QT-PCR and Western blot analysis, respectively. The caspase-7 mRNA levels were normalized to control values of β-actin and its protein levels were expressed as the ratio of caspase-7/β-actin bands density. n=3. Mean±SEM. ^aP>0.05, ^cP<0.01 vs media only; ^fP<0.01 vs PAR2-AP- or factor VIIa-stimulated alone.