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. 2011 Nov 21;33(1):120–126. doi: 10.1038/aps.2011.139

Figure 5.

Figure 5

Effects of EGCG on activation of ERK1/2 and NF-κB pathway in SW620 cells. The cells were pretreated with or without the indicated concentrations of EGCG for 15 min and then incubated with PAR2-AP (100 μmol/L) or factor VIIa (10 nmol/L) for indicated time. The above EGCG was kept in media. The cytoplasmic and nuclear lysates of the cells were collected and subjected to Western analysis with antibodies to total and phosphorylated ERK1/2 (A) and to NF-κB/p65 as well as Histon H3 (B). n=3. Mean±SEM. aP>0.05, bP<0.05, cP<0.01 vs control; dP>0.05, eP<0.05, fP<0.01 vs PAR2-AP- or factor VIIa-stimulated alone.