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. 2014 Mar 21;53(16):2605–2614. doi: 10.1021/bi4015488

Figure 7.

Figure 7

Inhibition by insulin of the formation of amyloid by proIAPP1–48 in the presence and absence of HS in buffer without HFIP. (A) Inhibition in the absence of HS monitored by thioflavin-T assays. Data are plotted as time normalized by the T50 value of proIAPP1–48 in the absence of insulin: black, proIAPP1–48; red, proIAPP1–48 and insulin in a 20:1 ratio. (B) Inhibition in the presence of HS monitored by thioflavin-T assays. Data are plotted as time normalized by the T50 value of proIAPP1–48 in the presence of HS, but in the absence of insulin. The same color coding is used here as in panel A. (C) TEM image of proIAPP1–48 in the absence of HS. (D) TEM image of a 20:1 mixture of proIAPP1–48 and insulin in the absence of HS. (E) TEM image of proIAPP1–48 in the presence of HS. (F) TEM image of a 20:1 mixture of proIAPP1–48 and insulin in the presence of HS. Scale bars represent 100 nm. Aliquots were removed at the end of each experiment for TEM analysis. The kinetic experiments were conducted in 20 mM Tris-HCl (pH 7.4) without stirring at 25 °C. The proIAPP1–48 concentration was 16 μM. The HS concentration was 1.3 μM (when present).