Table 2. Spectral Features and Lifetimes of the TA Components for KillerRed, mRFP, and DsRed^a.

	Killer Red	mRFP	DsRed
steady-state absorption	λmax = 585 nm (2.12 eV)	λmax = 585 nm (2.12 eV)	λmax = 561 nm (2.21 eV)
steady-state emission	λmax = 609 nm (2.04 eV)	λmax = 609 nm (2.04 eV)	λmax = 595 nm (2.08 eV)
light-minus-dark spectrum	λbleach = 585 nm (2.12 eV)	λbleach = 585 nm (2.12 eV)	λbleach = 560 nm (2.21 eV)
	shoulder ∼640 nm	shoulder ∼640 nm	shoulder ∼660 nm
	λmax = 735 nm (1.69 eV)	λmax = 722 nm (1.72 eV)	λmax = 745 nm (1.66 eV)
multiexponential decomposition of a	τ2 = 290 μs:	τ2 = 270 μs:	τ2 = 910 μs:
laser flash-induced transient absorption	λmax = 731 nm (1.70 eV)	λmax = 722 nm (1.72 eV)	λmax = 740 nm (1.68 eV)
decay (2 μs–4 s span)
subpicosecond transient abs decays	τ = 1.45 ns	τ = 1.8 ns	τ = 3.9 ns
fluorescence lifetimes	τ = 1.6 ns	τ = 2.0 ns	τ = 3.6 ns

^aAll data are taken in PBS buffer, pH 7.5 at 25 °C.