. Author manuscript; available in PMC: 2014 May 5.

Published in final edited form as: Anticancer Agents Med Chem. 2012 Dec;12(10):1264–1280. doi: 10.2174/187152012803833107

Table 5.

Summary of apparent kinetic parameters of genistein glucuronidation in different rat microsomes and human UGT isoforms

Kinetic parameters	Sprague-Dawley rats					Human UGT isoforms
Kinetic parameters	Duodenum	Jejunum	Ileum	Colon	Liver	UGT1A1	UGT1A8	UGT1A9	UGT1A10
V_max1 (nmol/min/mg)	1.445	3.171	1.423	0.435	3.713	NA	1.24	2.09	12.34
K_m1 (μM)	4.04	6.628	8.239	3.186	23.6	NA	2.24	1.29	1.04
V_max1 /K_m1 (ml/min/mg)	0.357	0.478	0.172	0.136	0.157	NA	1.81	0.62	0.08
V_max2 (biphasic) (nmol/min/mg)			0.041	1.59E3	0.826
K_m2 (μM)			7.994	2.74E3	0.71E-7
V_max-0 (autoactivation) (nmol/min/mg)						NA	0.01
V_max-d (autoactivation) (nmol/min/mg)						NA	1.23
R²	0.998	0.995	0.963	0.995	0.989	NA	0.96	0.98	0.99
AIC	−55.67	−19.13	−21.85	−53.61	−17.9	NA	−7.98	−26.91	−25.23

Data come from reference [70, 87, 91].

NA Eadie-Hofstee plot of genistein by UGT1A1 does not fit any known model.