Table 2.

Intra-day precision analysis for absolute quantification of human UGT1A1. Two batches of pooled, individual HLMs and UGT1A1 Supersomes™ were used in the determinations (n=3).

			UGT1A1 (pmol/mg Protein)
			Peptide 1		Peptide 2
			MRM1	MRM2	MRM1	MRM2
Batch 1	pHLM	Average	9.9	12.2	11.0	13.5
		% C.V.	1.5	1.0	1.0	0.4
	Supersome™	Average	179.0	180.2	175.6	198.6
		% C.V.	3.4	1.6	1.0	2.0
	HLM 442	Average	5.6	8.1	8.6	9.9
		% C.V.	5.0	2.2	1.2	2.6
	HLM 743	Average	18.4	20.5	22.5	26.2
		% C.V.	3.7	4.2	3.3	5.4
	HLM 751	Average	18.4	20.8	20.1	23.9
		% C.V.	2.7	0.8	1.5	2.0
Batch 2	pHLM	Average	8.6	9.0	3.1	5.1
		% C.V.	4.4	2.0	3.3	3.8
	Supersome™	Average	170.0	176.6	100.4	111.4
		% C.V.	3.4	1.6	1.0	2.0
	HLM442	Average	4.6	4.8	1.6	3.1
		% C.V.	5.8	3.6	3.9	4.8
	HLM 743	Average	16.8	17.2	9.7	12.2
		% C.V.	3.9	5.0	4.5	6.6
	HLM 751	Average	17.5	18.0	8.6	11.3
		% C.V.	2.7	0.9	2.1	2.4