Table 3. Gender-dependent sulfation rates of three flavones (7-MHF, 6-MHF and 4'-MHF) in liver S9 fractions prepared from the same animal but different genders.

Three different concentrations (5 μM, 10 μM, 40 μM) were used in the experiment (n = 3). Sulfation rates were calculated as nmol min⁻¹ mg⁻¹ protein.

Sulfation rates (nmol/min/mg, Mean±SD)		Mouse		Rat		Dog
		Female	Male	Female	Male	Female	Male
7-MHF	5μM	0.0202±0.0006	0.0228±0.0003	0.0855±0.0015*	0.1354±0.0018	0.1557±0.0023	0.1560±0.0061
	10μM	0.0250±0.0008*	0.0220±0.0008	0.0792±0.0013*	0.1254±0.0032	0.1685±0.0067	0.1678±0.0062
	40μM	0.0277±0.0007*	0.0213±0.0006	0.1030±0.0022*	0.1956±0.0122	0.1153±0.0174*	0.1529±0.0041
6-MHF	5μM	0.0009±0.0001*	0.0005±0.0001	0.0028±0.0002*	0.0048±0.0004	0.0582±0.0003	0.0576±0.004
	10μM	0.0012±0.0001*	0.0004±0.0001	0.0041±0.0002*	0.0066±0.0001	0.0703±0.0049	0.0734±0.0003
	40μM	0.0016±0.0002*	0.0007±0.0001	0.0061±0.0004*	0.0099±0.0002	0.1365±0.0091*	0.1150±0.0112
4'-MHF	5μM	0.0564±0.0098*	0.0402±0.0019*	0.0130±0.0010*	0.0329±0.0065	0.0894±0.0029	0.0913±0.0021
	10μM	0.0453±0.0012*	0.0322±0.0032	0.01780±0.0008*	0.0429±0.0013	0.0958±0.0028	0.1001±0.0048
	40μM	0.0475±0.0021*	0.0231±0.0003	0.0148±0.0025*	0.0327±0.0035	0.1944±0.0211	0.1873±0.0017

Significant differences are marked by the *symbol (p < 0.05, Kruskall–Wallis test).