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. 2012 Feb 3;33(2):261–270. doi: 10.1038/aps.2011.156

Figure 2.

Figure 2

Effects of MPSP-001 on cell cycle distribution and cell death. (A) The concentration effects of MPSP-001 on cell cycle progression of HeLa cells. HeLa cells were treated with different concentrations of MPSP-001 for 16 h. Then, the cells were fixed and stained with PI and analyzed by flow cytometry. Percentages of cells in different phases were shown. The data are representative of three independent experiments. (B) The time effects of MPSP-001 on cell cycle progressions of HeLa cells. HeLa cells were treated with 5 μmol/L MPSP-001 for different time. The cells were then fixed and stained with PI, and analyzed by flow cytometry. Percentages of cells in different phases were shown. The data are representative of three independent experiments. (C) MPSP-001 induced apoptosis with the cleavage of PARP. The time (left panel) and concentration (right panel) effects of MPSP-001 on cell apoptosis of HeLa cells. Protein samples were separated by SDS-PAGE for immunoblot analysis using antibody against PARP and GAPDH were stained as the internal cytosolic control.