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. 2012 Feb 3;33(2):261–270. doi: 10.1038/aps.2011.156

Figure 3.

Figure 3

Effects of MPSP-001 on mitotic spindle formation and tubulin disruption. (A) Illustrations of the effects of MPSP-001 on the mitotic spindle in HeLa cells (e-h) using immuno-fluorescence microscopy technique. HeLa cells grown in log phase were treated with 5 μmol/L MPSP-001 for 16 h, followed by fixation and immunofluorescence staining for tubulin (white) and DNA (blue). Examples of a normal interphase (a), normal prometaphase (b), normal metaphase (c) and normal telophase (d) were shown. Examples of a damaged interphase (e) and various forms of abnormal mitoses include a tripolar prometaphase (f), a mutipolar metaphase (g) and an abnormal bipolar metaphase with lagging chromosomes or chromosomes not aligned to the metaphase plate (h) were shown (×600). (B) Statistical analysis of the effects of MPSP-001 on the mitotic spindles in HeLa cells. (C) Disruption of tubulin by MPSP-001, colchicine, and paclitaxel in HeLa cells and tubulin-GFP-HeLa cells (over-expression of the fusion protein of tubulin and GFP). HeLa cells and tubulin-GFP-HeLa cells grown in log phase were treated with 0.1% DMSO (a, f), 250 nmol/L Taxol (b, g), 100 nmol/L colchicine (c, h), 100 nmol/L vincristine (d, i) and 5 μmol/L MPSP-001 for 16 h, followed by fixation and immunofluorescence staining for tubulin (In Tubulin-GFP-HeLa cells, immunofluorescence staining was omitted) .Then the morphology of interphase tubulin (white) was observed (×600).