Table 1. Primers sequences for PCR, annealing temperature, and predicted size.
Primers | Sequences | Annealing temperature (°C) | Predicted size (bp) |
---|---|---|---|
eNOS | 5′-TAACACAGACAGTGCAGGGG-3′ | 62 | 380 |
5′-CCTGGAACATCTTCCGTCTG-3′ | |||
tPA | 5′-AGAGAGGTTTCCACCCCATC-3′ | 58 | 248 |
5′-CTGTCCAGTCAGGGAGCTGT-3 | |||
GAPDH | 5′-TGCACCACCAACTGCTTAGC-3′ | 58 | 191 |
5′-GGCATGGACTGTGGTCATGAG-3′ |