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. 2012 May 4;33(5):644–651. doi: 10.1038/aps.2012.2

Table 1. Primers sequences for PCR, annealing temperature, and predicted size.

Primers Sequences Annealing temperature (°C) Predicted size (bp)
eNOS 5′-TAACACAGACAGTGCAGGGG-3′ 62 380
  5′-CCTGGAACATCTTCCGTCTG-3′    
tPA 5′-AGAGAGGTTTCCACCCCATC-3′ 58 248
  5′-CTGTCCAGTCAGGGAGCTGT-3    
GAPDH 5′-TGCACCACCAACTGCTTAGC-3′ 58 191
  5′-GGCATGGACTGTGGTCATGAG-3′