Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Sirt1 protein was overexpressed by transfecting MC3T3-E1 cells with Ad-Sirt1 adenovirus. MC3T3-E1 cells were transfected with Ad-Sirt1 or Ad-lacZ for 12 h. The expression of Sirt1 (A) was assayed by Western blotting analysis, and deacetylase activity (B) of overexpressed Sirt1 was measured using fluorogenic substrate. GAPDH, glyceraldehyde phosphate dehydrogenase. bP<0.05 vs Ad-lacZ cells.
