Figure 3.

The effect of Sirt1 overexpression on TNF-α-induced cell injury in MC3T3-E1 cells. After successfully transfected with Ad-Sirt1 or Ad-lacZ, two groups of cells were stimulated with TNF-α, the apoptosis (Western blot results and responding quantification of cleaved caspase-3 and caspase-3, A), ALP activity (B), and mRNA levels of Runx2 (C) and osteocalcin (D) were assessed. ALP, alkaline phosphatase; Runx2, runt-related transcription factor 2. ^bP<0.05 vs Ad-lacZ cells. ^eP<0.05 vs TNF-α-treated Ad-lacZ cells.