Skip to main content
. 2012 Mar 26;33(5):668–674. doi: 10.1038/aps.2011.189

Figure 6.

Figure 6

The effect of resveratrol on TNF-α-induced cell injury in MC3T3-E1 cells. Cells were treated with α-MEM (control), TNF-α (5 ng/mL), TNF-α+Resveratrol (10 μmol/L, a Sirt1 activator), TNF-α+Resveratrol+EX-527 (5 μmol/L, a Sirt1 inhibitor). Sirt1 deacetylase activity (A), Luciferase activity (B), supernatant NOx content (C), ALP activity (D), and expressions of Runx2 (E) and osteocalcin (F), apoptosis (Western blot results and responding quantification of cleaved caspase-3 and caspase-3, G), were assessed. NOx, nitrite and nitrate; ALP, alkaline phosphatase; TNF-α, tumor necrosis factor alpha; Res, Resveratrol. bP<0.05 vs control. eP<0.05 vs only TNF-α-treated cells. hP<0.05 vs TNF-α+Resveratrol-treated cells.