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Results of IHC staining for Shh, Gli1, p-ERK1/2, and MMP-9 from 86 HCC liver tissues including 37 cases with metastasis at 200× magnification. Representative images are displayed. Expression of Shh was defined as the cytoplasmic and plasmalemmal staining. Expression of MMP-9 was defined as cytoplasmic staining. Gli1, and p-ERK1/2 were located in cytoplasm and/or nucleus. (A) Normal liver tissues; (B) Non-metastatic HCC liver tissues; (C) Metastatic HCC liver tissues.
