Figure 3.

Concentration-dependent block by 18β-GA of WT I_Na,P (A), ΔKPQ I_Na,P (B), and I_Na,L (C). I_Na was elicited by a depolarizing pulse from a holding potential of -120 mV to -30 mV for 500 ms at 0.1 Hz. Representative traces (upper panels) were superimposed before (control) and during perfusion of 18β-GA (1–100 μmol/L). (B, C) are traces from the same oocyte on an expanded scale. Summarized dose-response data (lower panels) fitted with the Hill equation. IC₅₀ values and the Hill coefficient are provided in the figure. n=6–27 oocytes/point.