Table 1.

Clinical-demographical characteristics of NSCLC dataset

	Adenocarcinoma-mRNA (complete dataset)		Adenocarcinoma-mRNA (For survival analysis)		Squamous Carcinoma-mRNA (complete dataset)	Squamous Carcinoma-mRNA (For survival analysis)	Adenocarcinoma -protein (complete dataset)	Squamous Carcinoma-protein (complete dataset)
	Mayo Clinic dataset (DASL, N=77)	TCGA dataset (RNA-seq V2, N=288)	Mayo Clinic dataset (DASL, N=77)	TCGA dataset (RNA-seq V2, N=188)	TCGA dataset (RNA-seq V2, N=220)	TCGA dataset (RNA-seq V2, N=169)	Daping Hospital (IHC, N=75)	Daping Hospital (IHC, N=75)
Age
N	77	269	77	188	212	169	74	75
Mean (SD)	67.8(12.9)	65.6(9.7)	67.8(12.9)	64.8(10.1)	67.5(8.4)	67.8(8.2)	60.9	64.4(8.1)
Gender
Male	14(18.2%)	133(46.2%)	14(18.2%)	94(50.0%)	161(73.2%)	124(73.4%)	41(54.7%)	71(94.7%)
Female	63(81.8%)	155(53.8%)	63(81.8%)	94(50.0%)	59(26.8%)	45(26.6%)	34(45.3%)	4(5.3%)
Tumor stage
I	51(66.2%)	147(51.0%)	51(66.2%)	95(50.5%)	114(51.8%)	91(53.8%)	27(36.0%)	27(36.0%)
II	9(11.7%)	61(21.2%)	9(11.7%)	39(20.7%)	53(24.1%)	37(21.9%)	16(21.3%)	28(37.3%)
III	17(22.1%)	58(20.1%)	17(22.1%)	41(21.8%)	46(20.9%)	36(21.3%)	27(36.0%)	18(24.0%)
IV	0 (0.0%)	16(5.6%)	0 (0.0%)	10(5.3%)	4(1.8%)	3(1.8%)	5(6.7%)	2(2.7%)
Unknown	0(0.0%)	6(2.1%)	0(0.0%)	3(1.6%)	3(1.4%)	2(1.2%)	0(0.0%)	0(0.0%)
Smoking
Never smoker	77(100%)	36(12.5%)	77(100%)	0(0.0%)	10(4.5%)	0(0.0%)	0(0.0%)	0(0.0%)
Current/Ever smoker	0(0.0%)	240(83.3%)	0(0.0%)	188(100%)	204(92.7%)	169(100%)	0(0.0%)	0(0.0%)
Unknown	0(0.0%)	12(4.2%)	0(0.0%)	0(0.0%)	6(2.7%)	0(0.0%)	75(100%)	75(100%)

Note: DASL -(cDNA-mediated Annealing Selection extension and Ligation) microarray

RNA-seq – RNA sequencing

IHC –immunohistochemistry