Figure 3. Effect of treatment with S-8 on maintenance of preformed biofilms.

C. albicans biofilms were formed for 24 h followed by 24-h treatment with 1, 4 and 16 µg/ml S-8. Morphology of C. albicans in pre-formed biofilms exposed to S-8 was visualized using phase-contrast microscopy. A. Untreated control. B, C and D. Preformed biofilms exposed to 1, 4 and 16 µg/ml S-8, respectively. Magnification: 100X. At least four random fields were observed. Three independent experiments were performed. E. Biofilm metabolic activity was estimated using the XTT reduction assay. The XTT values of the untreated control were set to 100%. The results are presented as means ± SD of three independent experiments. *Significantly lower than the value for the untreated control (P<0.05).