Table 1. BTV Real time RT-PCR threshold cycle (Ct) values and corresponding copy number/ml in blood and swab material collected from 3 goats (GT01–GT03) experimentally infected with BTV-26 and 3 direct contact goats (GT04–GT06).

Sample	dpi	GT01		GT02		GT03		GT04		GT05		GT06
		Ct	copy number/ml	Ct	copy number/ml	Ct	copy number/ml	Ct	copy number/ml	Ct	copy number/ml	Ct	copy number/ml
Blood	0	No Ct	−	No Ct	−	No Ct	−	No Ct	−	No Ct	−	No Ct	−
Blood	2	No Ct	−	45.3	2.59×102	44.3	9.78×102	No Ct	−	No Ct	−	No Ct	−
Blood	4	34.5*	5.06×103	32.2*	1.32×105	33.6*	5.31×104	No Ct	−	No Ct	−	No Ct	−
Blood	7	25.3*	2.36×106	23.4*	4.80×107	25.2*	1.42×107	No Ct	−	No Ct	−	No Ct	−
Ocular Swab	7	No Ct	-	37.9*	2.98×103	No Ct	-
Nasal Swab	7	No Ct	-	No Ct	-	39.4	1.10×103
Blood	9	23.1*	1.17×107	21.4*	1.73×108	22.6*	7.96×107	No Ct	−	No Ct	−	No Ct	−
Ocular Swab	9	No Ct	-	No Ct	-	No Ct	-
Nasal Swab	9	37.1	5.03×103	36.7	6.82×103	36.5	7.68×103
Blood	11	22.6*	1.51×107	22.5*	8.38×107	22.2*	1.01×108	No Ct	−	No Ct	−	No Ct	−
Ocular Swab	11	No Ct	-	No Ct	-	No Ct	-
Nasal Swab	11	38.6	1.88×103	37.1	4.98×103	33.9	4.40×104
Blood	14	22.8*	1.29×107	24.3*	2.55×107	22.9*	6.14×107	No Ct	−	No Ct	−	No Ct	−
Ocular Swab	14	No Ct	-	No Ct	-	No Ct	-
Nasal Swab	14	34.9	2.27×104	37.0	5.64×103	35.9	1.11×104
Blood	16	25.1*	2.65×106	28.8*	1.31×106	24.7*	2.07×107	No Ct	−	No Ct	−	No Ct	−
Ocular Swab	16	No Ct	-	No Ct	-	No Ct	-
Nasal Swab	16	No Ct	-	38.5	2.08×103	37.6	3.60×103
Blood	18	26.1*	1.33×106	29.5*	8.09×105	25.5*	1.17×107	No Ct	−	No Ct	−	No Ct	−
Ocular Swab	18	No Ct	-	No Ct	-	No Ct	-
Nasal Swab	18	39.2	1.24×103	No Ct	-	No Ct	-
Blood	21	28.4*	2.88×105	29.9*	6.03×105	30.1*	5.38×105	43.6	2.38×103	26.1*	6.30×106	28.7*	1.99×106
Ocular Swab	21	37.0	5.42×103	No Ct	-	No Ct	-
Nasal Swab	21	No Ct	-	No Ct	-	38.5	1.98×103
Blood	23	29.4*	1.45×105	30.1*	5.57×105	29.4*	8.71×105	28.3*	1.78×106	23.8*	7.07×107	23.0*	8.59×107
Ocular Swab	23	No Ct	-	No Ct	-	No Ct	-
Nasal Swab	23	36.7	6.53×103	33.5	5.47×104	37.1	5.04×103
Blood	25	30.9*	5.49×104	30.3*	4.79×105	30.4*	4.26×105	24.3*	2.44×107	25.0*	3.26×107	22.6*	1.14×108
Ocular Swab	25	No Ct	-	No Ct	-	No Ct	-	No Ct	-	No Ct	-	38.7	1.78×103
Nasal Swab	25	38.2	2.51×103	No Ct	-	No Ct	-	No Ct	-	34.7	2.59×104	38.4	2.15×103
Blood	28	29.5*	1.40×105	28.2*	1.91×106	30.1*	5.42×105	22.1*	1.13×108	22.8*	1.35×108	28.0*	3.34×106
Ocular Swab	28	No Ct	-	No Ct	-	No Ct	-	No Ct	-	No Ct	-	No Ct	-
Nasal Swab	28	No Ct	-	No Ct	-	38.1	2.69×103	35.3	1.72×104	36.5	7.63×103	32.2	1.30×105
Blood	30	30.1*	9.34×104	29.2*	1.05×106	30.7*	3.53×105	22.1*	1.15×108	24.3*	5.22×107	28.8*	1.87×106
Ocular Swab	30	No Ct	-	No Ct	-	No Ct	-	No Ct	-	No Ct	-	36.8	6.19×104
Nasal Swab	30	No Ct	-	No Ct	-	No Ct	-	37.3	4.45×103	39.3	1.21×103	38.5	1.96×103
Blood	32	30.1*	9.52×104	29.9*	6.13×105	31.0*	2.98×105	22.2*	1.04×108	24.5*	4.48×107	28.5*	2.38×106
Ocular Swab	32	No Ct	-	No Ct	-	No Ct	-	36.0	1.09×104	No Ct	-	No Ct	-
Nasal Swab	32	No Ct	-	No Ct	-	No Ct	-	No Ct	-	No Ct	-	No Ct	-

*Virus isolated directly on BHK.