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. Author manuscript; available in PMC: 2014 May 5.
Published in final edited form as: Vaccine. 2013 Jul 24;31(40):4314–4321. doi: 10.1016/j.vaccine.2013.07.025

Fig. 4.

Fig. 4

pWRG/SN-M(opt) DNA vaccine (gene gun) is immunogenic and protective in hamsters. Groups of 7–8 hamsters received 2 or 3 vaccinations with the pWRG/SN-M(opt) SNV DNA vaccine, 3 vaccinations with a negative control DNA vaccine, or no vaccine. (A) Sera collected were tested for SNV neutralizing antibodies by PRNT. Mean titers ± SE are shown. (B) Individual PRNT50 titers from sera collected on week 9 are presented with the GMT and 95% confidence interval depicted. The PRNT limit of detection was a titer of 20 (dashed lines). (C) Sera collected on week 16 (5 weeks postchallenge) were tested by ELISA for evidence of SNV infection. All prechallenge sera samples were negative by ELISA (data not shown).<indicates titer was below level of detection for the assay. *indicates antibody responses were statistically significant when compared to negative DNA vaccination controls.