Figure 1. EPA reduces aortic aneurysm formation.

Gross morphological and histological analyses of aortas were performed at 6 weeks after perivascular application of CaCl₂ to the infra-renal aorta. A. Representative images of in situ infra-renal aortas (demarcated by the broken lines) from mice in the sham-operated, control diet or EPA diet groups. B. Quantitative analysis of the maximal external aortic diameters of aortas. n = 4 for sham, n = 12 for control diet and EPA diet groups. C. Histological analysis by EVG staining, showing preserved aortic wall structure of the aorta from EPA diet group compared to the aorta from control diet group. Elastin breaks were also quantified. Scale bars, 200 µm (upper panels) and 50 µm (lower panels). n = 5 for sham, n = 11 for control diet, and n = 12 for EPA diet groups. Representative images of at least three independent experiments are shown in A and C. *P<0.05.