Table 1. Strains and plasmids used in this study.

S. Typhimurium strains	Relevant genotype	Method of construction	Reference
4/74	Parent strain	N/A	[50]
JH3486	4/74 ΔpfkA::Km ΔpfkB::Cm	λ Red mutagenesis	[12]
JH3537	4/74 ΔptsHI::Cm	λ Red mutagenesis	[12]
JH3536	4/74 ΔptsHIcrr::Cm	λ Red mutagenesis	[12]
JH3502	4/74 crr::Kn	λ Red mutagenesis	[12]
JH3504	4/74 ΔptsG::Cm	λ Red mutagenesis	[12]
JH3494	4/74 glk::Kn	λ Red mutagenesis	[12]
JH3541	4/74 ΔmanXYZ	λ Red mutagenesis	[12]
AT1011	4/74 ΔptsG::Cm, ΔmanXYZ	λ Red mutagenesis	[12]
AT1012	4/74 ΔptsG::Cm, Δglk::Kn	λ Red mutagenesis	[12]
AT1014	4/74 ΔptsG::Cm, ΔmanXYZ, Δglk::Kn	λ Red mutagenesis	[12]
JH3469	4/74 ΔaceA	λ Red mutagenesis	[14]
JH3487	4/74 Δpps, ΔpckA::Kn	λ Red mutagenesis	This study
Plasmids
pKD46	λ Red recombinase expression plasmid	N/A	[17]
pKD3	CmR resistance cassette-containing plasmid	N/A	[17]
pKD4	KnR resistance cassette-containing plasmid	N/A	[17]
pCP20	FLP-recombinase expression plasmid	N/A	[17]
pWKS30	ApR low-copy-number vector, pSC101 origin of replication	N/A	[51]
pWKS30::pfkA	ApR low-copy-number vector, pSC101 origin of replication, expresses pfkA	N/A	[12]