Table 1. Performance of HIV-1/2 viral stocks in real-time RT-qPCR and HIV-1 p24 antigen tests.
| HIV Viral Stock Information | HIV-2 Tests | HIV-1 Tests | |||||
| NIAID | Isolate | Country | HIV-1/2 | PD | SM | Roche TaqMan | Perkin -Elmer |
| Catalog # | Name | of Origin | Subtype/Group | RNA copies/ml (log10) | RNA copies/ml (log10) | RNA copies/ml (log10) | p24 pg/ml |
| N/A | 91US_4 | USA | 1B | TND | TND | 5.12 | 236.0 |
| N/A | PB0012902206 | Cote d'Ivoire | 2B | 5.58 | 3.92 | TND | 0.2 |
| 3930 | CDC 310319 | Cote d'Ivoire | 2B | 5.60 | 4.02 | TND | 0.5 |
| 3932 | CDC 77618 | Cote d'Ivoire | 2A | 6.68 | 5.22 | TND | 0.0 |
| 3931 | CDC 310340 | Cote d'Ivoire | 2A | TND | TND | 5.34 | 230.0 |
| 3652 | CDC 310342 | Cote d'Ivoire | 2 UNK | TND | TND | 5.22 | 246.0 |
| 599 | CBL-23 | Gambia | 2A | 6.72 | 5.09 | – | – |
| 600 | CBL-20 | Gambia | 2A | 6.46 | 5.44 | – | – |
| 803 | MVP-15132 | Senegal | 2A | 6.58 | 5.17 | – | – |
| 1337 | D194 | Gambia | 2A | 6.13 | 4.90 | – | – |
| 3512 | 7924A | Cote d'Ivoire | 2A | 6.06 | 2.82 | – | – |
| 3513 | 60415K | Senegal | 2A | 5.81 | 4.99 | – | – |
| 3648 | CDC 310248 | Cote d'Ivoire | 2A | 4.83 | 4.00 | – | – |
| 3649 | CDC 310072 | Cote d'Ivoire | 2A | 6.33 | 5.19 | – | – |
Individual viral stocks identified as HIV-2 were obtained from the NIAID AIDS Reagent and Reference Repository and SeraCare, Inc. The HIV-1 subtype and HIV-2 group identification is based upon data sheets provided by NIAID and from publications. An HIV-1 Subtype B isolate from the United States (91US_4) was used as an HIV-1 control. Viral RNA was extracted and tested in HIV-2 real-time RT-qPCR assays. Viral isolates selected for testing in the Roche COBAS AmpliPrep/COBAS TaqMan HIV-1 v2.0 quantitative RNA assay and the Perkin-Elmer p24 Antigen test included the two viral isolates that were not amplified in the HIV-2 real-time RT-qPCR assays, three HIV-2 viral isolates that amplified well and the HIV-1 91US_4 isolate. Although the HIV-2 RT-qPCR assays were not optimized, the HIV-2 amplifications were robust for the majority of the isolates tested as reflected in the HIV-2 RNA concentrations reported based upon relative values extrapolated from the NIH-Z standard for each primers/probe set. The CDC 310340 and CDC 310342 viral stocks were not amplified in HIV-2 RT-qPCR assays but were quantified and reactive in HIV-1 specific tests. PD = Primer Design LTD HIV-2 PCR Kit primers/probe. SM = Delarue et al [12] primers/probe set. TND = Target Not Detected. UNK is unknown group. Viral isolate was not tested (−).