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. 2014 May 5;9(5):e96610. doi: 10.1371/journal.pone.0096610

Figure 2. c-Jun promoted expression of SPRR3, but TAM67 suppressed expression of SPRR3.

Figure 2

(A) KYSE450 cells were transiently transfected with pCMV-c-Jun. After 48 hours, cells were lysed, and analyzed by Western blot. (B) KYSE450 cells were transfected with pCMV-c-Jun and (or) pcDNA3.1-TAM67. After 48 hours, cells were lysed, and detected by Western blot. (C) TAM67 suppresses AP1 factor-dependent promoter activity. KYSE450 cells were cotransfected with the promoter construct of SPRR3 and pCMV-c-Jun or (and) pcDNA3.1-TAM67. pRL-SV40 Renilla was used for normalization of transfection. Data shown are Mean ± SD from multiple independent experiments (*: P< 0.05). (D) MAPK inhibitors suppressed TPA-triggered activation of c-Jun. KYSE450 cells were pretreated with 20 µM PKC inhibitor (GF109203X), JNK inhibitor (SP600125) or MEK inhibitor (PD98059) for 1 hour respectively, and then treated with 100 ng/ml TPA for 8 hours. Cells were lysed. The expression levels of c-Jun and SPRR3 were examined by Western blot. β-actin was used as a loading control.