Figure 4. Time course and concentration dependence of effect of arginine on transgene expression.

Mycoplasma-infected B⁰AT1-TMEM27 overexpressing MDCK cells were cultivated in arginine-free DMEM supplemented with 45, 180 or 720 µM arginine for the indicated times. A: Real-time PCR quantification of B⁰AT1 mRNA expression. mRNA levels were standardized to β-actin and normalized to time 0. B: Representative Western blotting of B⁰AT1 expression. C: the intensity of B⁰AT1 immunoreactive bands was quantified, standardized to β-actin and normalized to 0 h. Data are represented as mean ± SEM (n = 3). Groups were compared by two-way ANOVA followed by Bonferroni post-test; *** p≤0.001, * p≤0.05.