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. 2014 May 5;9(5):e96786. doi: 10.1371/journal.pone.0096786

Figure 2. ATP levels under different metabolic conditions and the effect of OXPHOS inhibition on glycolytic flux.

Figure 2

RAW 264.7 cells were incubated for the indicated time intervals with control (25 mM glucose) medium, or medium containing 2.5 µM oligomycin and 25 mM glucose (A,D,E), 10 mM 2-DG and 25 mM glucose (B), or 10 mM galactose and no glucose (C). Intracellular ATP concentrations and total cellular protein were measured in PCA cell extracts (A,B,C). Glucose consumption (D) and lactate production (E) were measured in medium supernatants during the first 6 hours (0–6 h), the last 6 hours (18–24 h), as well as the whole 24 hours (0–24 h) of treatment. Data represent means ± SEM of three experiments performed in triplicate. (*p<0.05, unpaired t-test).