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. 2014 May 5;9(5):e96786. doi: 10.1371/journal.pone.0096786

Figure 4. Effect of glucose deprivation and glycolysis or OXPHOS inhibition on morphology of RAW 264.7 cells.

Figure 4

Cells were seeded on glass coverslips, incubated in control medium or medium containing 2.5 µM oligomycin and 25 mM glucose (A–F), 10 mM 2-DG and 25 mM glucose (G–J), 10 mM galactose and no glucose (M–P), or 1 mM glucose and 10 mM galactosel (Q–T) for the indicated time periods and stimulated overnight with LPS or left unstimulated. Coverslips were fixed and subjected to scanning electron microscopy. The number of filopodia extending radially from the cell surface was determined for control cells and cells treated for 24 hours with oligomycin, in the presence and absence of LPS (K). The average cell circumference was determined for cells in control medium or medium containing 10 mM galactose, or 1 mM glucose and 10 mM galactose (L). (***p<0.001, unpaired t-test). (Bar = 10 µm).