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. 2014 May 5;9(5):e96681. doi: 10.1371/journal.pone.0096681

Figure 5. IFN-γ stimulates double-membrane autophagosome formation.

Figure 5

5A) Electron microscopy of non-CF macrophage infected with k56-2 only for 24 hours. White arrow indicates double membrane formation indicative of autophagosomes. 5B) EM of non-CF macrophage treated with IFN-γ for 24 hours. 5C) EM of CF macrophage infected with k56-2 only. Black arrow indicates single membrane vacuole. 5D) EM of CF macrophage treated with IFN-γ for 24 hours. White arrow indicates double membrane formation. Pictures are marked with 500 nm marker.